منابع مشابه
Rabbit Brain Phosphofructokinase
A procedure was developed for the purification of phosphofructokinase from rabbit brain extracts. The enzyme was purified more than loo-fold with a yield of greater than 50%. It was found to have the same distribution of multiple molecular forms as found in the total brain extract, including one molecular species that is different from the more extensively studied muscle (heart) and liver (eryt...
متن کاملMuscle phosphofructokinase deficiency. Biochemical and immunological studies of phosphofructokinase isozymes in muscle culture.
Muscle cultures from three unrelated patients with muscle phosphofructokinase (PFK; EC 2.7.1.11) deficiency (Glycogenosis type VII; Tarui disease) had normal PFK activity and normal morphology. Chromatographic and immunological studies showed that normal muscle cultures express all three PFK subunits, M (muscle-type), L (liver-type), and P (platelet-type) and contain multiple homotetrameric and...
متن کاملRabbit liver phosphofructokinase. Comparison of some properties with those of muscle phosphofructokinase.
A relatively simple procedure was devised for the purification of phosphofructokinase from rabbit liver extracts. The enzyme was purified more than 2600-fold with a yield of close to 50%. Liver phosphofructokinase migrates faster on zone electrophoresis than rabbit skeletal muscle phosphofructokinase. It also differs from muscle enzyme in stability, molecular weight, and kinetic properties. The...
متن کاملIsolation and characterization of phosphofructokinase C from rabbit brain.
Phosphofructokinase from rabbit brain consists of hybrids of the A, B, and C isozymes. Phosphofructokinase C was isolated from a purified mixture of such hybrids in a 2-step procedure. In the first step, phosphofructokinase B was removed by chromatography on DEAE-Sephadex. In the second step, subunits of phosphofructokinases A and C were separated by dissociation at pH 5.0 followed by chromatog...
متن کاملPurification and Some Properties of Rabbit Skeletal Muscle Phosphofructokinase.
In spite of an abundant literature (1-15) indicating that phosphofructokinasel plays a key role in the regulation of carbohydrate metabolism, few attempts have been made to purify the enzyme (4, 17, 18) since the subject of P-fructokinase was last reviewed (19). The apparent instability of the enzyme makes purification difficult, and a number of suggestions have been offered to explain this pro...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44336-6